» Leonardo :: The courageous leader and devoted student of martial arts. He is the eldest of the four and the most skilled fighter of the four. Leonardo wears a blue mask and wields two katanas. Leonardo was named after the Italian polymath, scientist, engineer, inventor, anatomist, and painter, Leonardo Da Vinci.
» Donatello :: The brilliant scientist, inventor, engineer, and technological genius. He is perhaps the least violent Turtle, preferring to use his intellect to solve conflicts. He wears a purple mask and wields the bo staff. He is named after the sculptor Donatello.
» Raphael :: The team's bad boy, he has an aggressive nature and seldom hesitates to throw the first punch. He is an intense fighter. His personality can be alternately fierce and sarcastic, and oftentimes delivers deadpan humor. Still, he is intensely loyal to his brothers and sensei. He is good friends with Casey Jones, after having to meet him one night on patrol and challenging him to a fight. The two have since frequently patrolled together. Raphael wears a red mask and wields a pair of sai. He is named after the Italian painter and architect of the High Renaissance, Raphael Sanzio.
» Michelangel :: The easy-going and free-spirited Mikey provides much of the comic relief. While he loves to relax, this Turtle also has an adventurous and creative side. He wears an orange mask and wields nunchaku. He is the youngest of the four. He is named after Michelangelo Buonarroti. His name was originally misspelled "Michaelangelo" by Peter Laird and Kevin Eastman at the time they were using a typewriter and did not own a dictionary.
» Master Splinter :: The Turtles' sensei and adoptive father, Splinter is a mutant rat who learned the ways of ninjutsu from his own master, Hamato Yoshi. In the 1987 series, Splinter was Hamato Yoshi himself mutated into a rat.
» Shredder :: A villainous ninjutsu master called Oroku Saki. In every incarnation of the TMNT franchise, he has been the archenemy of Splinter and the Turtles. Shredder prefers to use his armor instead of weapons. He is also the leader of the Foot Clan.
» April O'Neil :: A former lab assistant to the mad scientist Baxter Stockman, April is the plucky human companion of the Turtles. April first met up with the Turtles when they saved her from Baxter's Mouser robots. She embarks on many of the Turtles' adventures and aids them by doing the work that the Turtles themselves cannot do in public. In the 1987 series, and subsequent three films, April was a television news reporter.
» Casey Jones :: A vigilante who has become one of the Turtles' closest allies as well as a love interest to April. Casey first met up with the Turtles after having a fight with Raphael. Casey fights crime with an assortment of sporting goods (baseball bats, golf clubs, hockey sticks, cricket bat etc.) while wearing a hockey mask to protect his identity.
» Foot Soldiers
» Rat King
» Mondo Gecko
» Ray Fillet
» Wingnut & Screwloose
» Venus de Milo
» Dragon Lord